40nm AptamerREADY Gold Nanoparticle Conjugation Kit (3 reactions) reactant free gold nanoparticles Surface functionality: NHS-ester (~ 1

Surface functionality: NHS-ester (~ 1 NHS group/nm2) (10kDa PEG-spacer between gold surface and NHS-group)

Dilute the secondary antibody-HRP or streptavidin-HRP conjugate (if biotinylated detection primary antibody was used) to the desired concentration and volume in 1x ELISA Detection Diluent

TNF-alphacachectin

also known as vascular permeability factor (VPF)

40nm AptamerREADY Gold Nanoparticle Conjugation Kit (3 reactions) reactant free gold nanoparticles Surface functionality: NHS-ester (~ 1Cytodiagnostics AptamerREADY Gold Nanoparticle Conjugation Kits have been optimized for high efficiency one step conjugation of thiolated aptamers directly to the gold surface. The kit contains ready to use pre made mixtures. No activation, manipulation or time consuming "salt aging" steps are required for conjugation. Simply mix your thiol modified aptamer with the pre activated gold nanoparticles supplied in the kit. Conjugation of the aptamer is